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Infinicyt as software analysis in flow cytometric studies

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> 2017


Infinicyt publication Lahuerta JJ, Paiva B, Vidriales MB, Cordón L, Cedena MT, Puig N, Martinez-Lopez J, Rosiñol L, Gutierrez NC, Martín-Ramos ML, Oriol A, Teruel AI, Echeveste MA, de Paz R, de Arriba F, Hernandez MT, Palomera L, Martinez R, Martin A, Alegre A, De la Rubia J, Orfao A, Mateos MV, Blade J, San-Miguel JF; GEM (Grupo Español de Mieloma)/PETHEMA (Programa para el Estudio de la Terapéutica en Hemopatías Malignas) Cooperative Study Group. Depth of response in multiple myeloma: a pooled analysis of three PETHEMA/GEM clinical trials. J Clin Oncol 2017; doi.org/10.1200/JCO.2016.69.2517. [Epub ahead of print].

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Infinicyt publication Roshal M, Flores-Montero JA, Gao Q, Koeber M, Wardrope J, Durie BGM, Dogan A, Orfao A, Landgren O. MRD detection in multiple myeloma: comparison between MSKCC 10-color single-tube and EuroFlow 8-color 2-tube methods. Blood Adv 2017; 1(12): 728-32.

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Infinicyt publication Theunissen PMJ, Sedek L, De Haas V, Szczepanski T, Van Der Sluijs A, Mejstrikova E, Nováková M, Kalina T, Lecrevisse Q, Orfao A, Lankester AC, van Dongen JJM, Van Der Velden VHJ; EuroFlow Consortium. Detailed immunophenotyping of B-cell precursors in regenerating bone marrow of acute lymphoblastic leukaemia patients: implications for minimal residual disease detection. Br J Haematol 2017; doi.org/10.1111/bjh.14682. [Epub ahead of print].

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Infinicyt publication Criado I, Muñoz-Criado S, Rodríguez-Caballero A, Nieto WG, Romero A, Fernández-Navarro P, Alcoceba M, Contreras T, González M, Orfao A, Almeida J. Host virus and pneumococcus-specific immune responses in high-count monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia: implications for disease progression. Haematologica 2017; doi.org/10.3324/haematol.2016.159012. [Epub ahead of print].

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Infinicyt publication van der Velden VHJ, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, te Marvelde J, Teodosio C, Corral Mateos A, Kanderová V, van der Burg M, Van Hoof D, van Dongen JJM, Orfao A. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods 2017; doi.org/10.1016/j.jim.2017.03.011. [Epub ahead of print].

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Infinicyt publication Flores-Montero J, Sanoja-Flores L, Paiva B, Puig N, García-Sánchez O, Böttcher S, van der Velden VHJ, Pérez-Morán JJ, Vidriales MB, Garcia-Sanz R, Jimenez C, González M, Martinez-López J, Corral-Mateos A, Grigore GE, Fluxá R, Pontes R, Caetano J, Sedek L, del Cañizo MC, Bladé J, Lahuerta JJ, Aguilar C, Bárez A, García-Mateo A, Labrador J, Leoz, P, Aguilera-Sanz C, San Miguel J, Mateos MV, Durie B, van Dongen JJM, Orfao A. Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma. Leukemia 2017; 1-10; doi.org/10.1038/leu.2017.29. [Epub ahead of print].

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Infinicyt publication Mello FV, Alves LR, Land MG, Teodósio C, Sanchez ML, Bárcena P, Peres RT, Pedreira CE, Costa ES, Orfao A. Maturation-associated gene expression profiles along normal human bone marrow monopoiesis. Br J Haematol 2017; 176(3): 464-74.

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Infinicyt publication Theunissen P, Mejstrikova E, Sedek L, van der Sluijs-Gelling AJ, Gaipa G, Bartels M, Sobral da Costa E, Kotrova M, Novakova M, Sonneveld E, Buracchi C, Bonaccorso P, Oliviera E, te Marvelde JG, Szczepanski T, Lhermitte L, Hrusak O, Lecrevisse Q, Grigore GE, Fronkova E, Trka J, Brüggemann M, Orfao A, van Dongen JJM, van der Velden VHJ; EuroFlow Consortium. Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia. Blood 2017; 129(3): 347-57.

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> 2016


Infinicyt publication Paiva B, Cedena MT, Puig N, Arana P, Vidriales MB, Cordon L, Flores-Montero J, Gutierrez NC, Martín-Ramos ML, Martinez-Lopez J, Ocio EM, Hernandez MT, Teruel AI, Rosiñol L, Echeveste MA, Martinez R, Gironella M, Oriol A, Cabrera C, Martin J, Bargay J, Encinas C, Gonzalez Y, Van Dongen JM, Orfao A, Bladé J, Mateos MV, Lahuerta JJ, San Miguel JF; Grupo Español de Mieloma/Programa para el Estudio de la Terapéutica en Hemopatías Malignas (GEM/PETHEMA) Cooperative Study Groups. Minimal residual disease monitoring and immune profiling in multiple myeloma in elderly patients. Blood 2016; 127(25): 3165-74.

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Infinicyt publication Oliveira E, Bacelar TS, Ciudad J, Ribeiro MCM, Garcia DRN, Sedek L, Maia SF, Aranha DB, Machado IC, Ikeda A, Baglioli BF, Lopez-Duarte N, Teixeira LAC, Szczepanski T, Silva MLM, Land MGP, Orfao A, Costa ES. Altered neutrophil immunophenotypes in childhood B cell precursor acute lymphoblastic leukemia. Oncotarget 2016; 7(17): 24664-76.

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Infinicyt publication Arroz M, Came N, Lin P, Chen W, Yuan C, Lagoo A, Monreal M, de Tute R, Vergilio JA, Rawstron AC, Paiva B. Consensus guidelines on plasma cell myeloma minimal residual disease analysis and reporting. Cytometry B Clin Cytom 2016; 90(1): 31-9.

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> 2015


Infinicyt publication Kalina T, Flores-Montero J, Lecrevisse Q, Pedreira CE, van der Velden VHJ, Novakova M, Mejstrikova E, Hrusak O, Böttcher S, Karsch D, Sędek Ł, Trinquand A, Boeckx N, Caetano J, Asnafi V, Lucio P, Lima M, Santos AH, Bonaccorso P, van der Sluijs-Gelling AJ, Langerak AW, Martin-Ayuso M, Szczepański T, van Dongen JJM, Orfao A. Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A 2015; 87(2): 145-56.

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Infinicyt publication van Dongen JJ, van der Velden VH, Brüggemann M, Orfao A. Minimal residual disease diagnostics in acute lymphoblastic leukemia: need for sensitive, fast, and standardized technologies. Blood 2015; 125(26): 3996-4009.

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> 2014


Infinicyt publication Sędek Ł, Bulsa J, Sonsala A, Twardoch M, Wieczorek M, Malinowska I, Derwich K, Niedźwiecki M, Sobol-Milejska G, Kowalczyk JR, Mazur B, Szczepański T. The immunophenotypes of blast cells in B-cell precursor acute lymphoblastic leukemia: how different are they from their normal counterparts? Cytometry B Clin Cytom 2014; 86(5): 329-39.

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Infinicyt publication Matarraz S, Paiva B, Díez-Campelo M, Bárrena S, Jara-Acevedo M, Gutiérrez ML, Sayagués JM, Sánchez ML, Bárcena P, Garrastazul MP, Berruezo MJ, Duran JM, Cerveró C, García-Erce JA, Florensa L, Méndez GD, Gutierrez O, del Cañizo MC, van Dongen JJ, San Miguel JF, Orfao A. Immunophenotypic alterations of bone marrow myeloid cell compartments in multiple myeloma patients predict for myelodysplasia-associated cytogenetic alterations. Leukemia 2014; 28(8): 1747-50.

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> 2013


Infinicyt publication Fernandez C, Santos-Silva MC, López A, Matarraz S, Jara-Acevedo M, Ciudad J, Gutierrez ML, Sánchez ML, Salvador-Osuna C, Berruezo MJ, Díaz-Arias JÁ, Palomo-Hernández AM, Colado E, González N, Gallardo D, Asensio A, García-Sánchez R, Saldaña R, Cerveró C, Carboné-Bañeres A, Gutierrez O, Orfao A. Newly diagnosed adult AML and MPAL patients frequently show clonal residual hematopoiesis. Leukemia 2013; 27(11): 2149-56.

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Infinicyt publication Gaipa G, Basso G, Biondi A, Campana D. Detection of minimal residual disease in pediatric acute lymphoblastic leukemia. Cytometry B Clin Cytom 2013; 84(6): 359-69.

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Infinicyt publication Sánchez-Abarca LI, Álvarez-Laderas I, Díez Campelo M, Caballero-Velázquez T, Herrero C, Muntión S, Calderón C, García-Guerrero E, Sánchez-Guijo F, del Cañizo C, San Miguel J, Pérez-Simón JA. Uptake and delivery of antigens by mesenchymal stromal cells. Cytotherapy 2013; 15(6): 673-8.

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> 2012


Infinicyt publication Sandes AF, Chauffaille M de L, Orfao A, Siufi GC, Silva MR, Yamamoto M. Association of myelodysplastic syndrome with CD5+, CD23+ monoclonal B-cell lymphocytosis. Clinics (Sao Paulo) 2012; 67(12): 1487-91.

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Infinicyt publication van Dongen JJM, Orfao A; EuroFlow Consortium. EuroFlow: Resetting leukemia and lymphoma immunophenotyping. Basis for companion diagnostics and personalized medicine. Leukemia 2012; 26(9): 1899-907.

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Infinicyt publication Matarraz S, Teodosio C, Fernandez C, Albors M, Jara-Acevedo M, López A, Gonzalez-Gonzalez M, Gutierrez ML, Flores-Montero J, Cerveró C, Pizarro-Perea M, Paz Garrastazul M, Caballero G, Gutierrez O, Mendez GD, González-Silva M, Laranjeira P, Orfao A. The proliferation index of specific bone marrow cell compartments from myelodysplastic syndromes is associated with the diagnostic and patient outcome. PLoS One 2012; 7(8): e44321; doi.org/10.1371/journal.pone.0044321.

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Infinicyt publication van Dongen JJM, Lhermitte L, Böttcher S, Almeida J, van der Velden VHJ, Flores-Montero J, Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepañski T, Kalina T, de Tute R, Brüggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin-Ayuso M, Hrusak O, Vidriales MB, Orfao A; EuroFlow Consortium. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012; 26(9): 1908-75.

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Infinicyt publication Dekking EHA, van der Velden VHJ, Varro R, Wai H, Böttcher S, Kneba M, Sonneveld E, Koning A, Boeckx N, Van Poecke N, Lucio P, Mendonça A, Sedek L, Szczepański T, Kalina T, Kanderová V, Hoogeveen P, Flores-Montero J, Chillón MC, Orfao A, Almeida J, Evans P, Cullen M, Noordijk AL, Vermeulen PM, de Man MT, Dixon EP, Comans-Bitter WM, van Dongen JJM; EuroFlow Consortium. Flow cytometric immunobead assay for fast and easy detection of PML-RARA fusion proteins for the diagnosis of acute promyelocytic leukemia. Leukemia 2012; 26(9): 1976-85.

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Infinicyt publication Kalina T, Flores-Montero J, van der Velden VH, Martin-Ayuso M, Böttcher S, Ritgen M, Almeida J, Lhermitte L, Asnafi V, Mendonça A, de Tute R, Cullen M, Sedek L, Vidriales MB, Pérez JJ, te Marvelde JG, Mejstrikova E, Hrusak O, Szczepański T, van Dongen JJM, Orfao A; EuroFlow Consortium. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia 2012; 26(9): 1986-2010.

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Infinicyt publication Subirá D, Serrano C, Castañón S, Gonzalo R, Illán J, Pardo J, Martínez-García M, Millastre E, Aparisi F, Navarro M, Dómine M, Gil-Bazo I, Pérez Segura P, Gil M, Bruna J. Role of flow cytometry immunophenotyping in the diagnosis of leptomeningeal carcinomatosis. Neuro Oncol 2012; 14(1): 43-52.

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> 2011


Infinicyt publication Matarraz S, Fernandez C, Albors M, Teodosio C, López A, Jara-Acevedo M, Cervero C, Caballero G, Gutierrez O, Orfao A. Cell-cycle distribution of different cell compartments in normal versus reactive bone marrow: a frame of reference for the study of dysplastic hematopoiesis. Cytometry B Clin Cytom 2011; 80(6): 354-61.

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Infinicyt publication Schmidt-Hieber M, Pérez-Andrés M, Paiva B, Flores-Montero J, Perez JJ, Gutierrez NC, Vidriales MB, Matarraz S, San Miguel JF, Orfao A. CD117 expression in gammopathies is associated with an altered maturation of the myeloid and lymphoid hematopoietic cell compartments and favorable disease features. Haematologica 2011; 96(2): 328-32.

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> 2010


Infinicyt publication Nieto WG, Teodosio C, López A, Rodríguez-Caballero A, Romero A, Bárcena P, Gutierrez ML, Langerak AW, Fernandez-Navarro P, Orfao A, Almeida J; Primary Health Care Group of Salamanca for the Study of MBL. Non-CLL-like monoclonal B-cell lymphocytosis in the general population: prevalence and phenotypic/genetic characteristics. Cytometry B Clin Cytom 2010; 78(Suppl 1): S24-34.

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Infinicyt publication Caraux A, Klein B, Paiva B, Bret C, Schmitz A, Fuhler GM, Bos NA, Johnsen HE, Orfao A, Perez-Andres M; Myeloma Stem Cell Network (MSCNET). Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- and CD138+ plasma cells. Haematologica 2010; 95(6): 1016-20.

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Infinicyt publication Westers TM, Alhan C, Chamuleau MED, van der Vorst MJDL, Eeltink C, Ossenkoppele GJ, van de Loosdrecht AA. Aberrant immunophenotype of blasts in myelodysplastic syndromes is a clinically relevant biomarker in predicting response to growth factor treatment. Blood 2010; 115(9): 1779-84.

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> 2009


Infinicyt publication Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B. An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization. Blood 2009; 114(25): 5173-81.

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Infinicyt publication Martín-Martín L, Almeida J, Hernández-Campo PM, Sánchez ML, Lécrevisse Q, Orfao A. Immunophenotypical, morphologic, and functional characterization of maturation-associated plasmacytoid dendritic cell subsets in normal adult human bone marrow. Transfusion 2009; 49(8): 1692-1708.

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Infinicyt publication de Souza TL, da Costa ES, Lopez DV, Borojevic R. Racemic Etodolac is cytotoxic and cytostatic for B-cell precursor acute lymphoblastic leukemia cells. Biomed Pharmacother 2009; 63(7): 548-51.

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> 2008


Infinicyt publication Rodríguez-Caballero A, García-Montero AC, Bárcena P, Almeida J, Ruiz-Cabello F, Tabernero MD, Garrido P, Muñoz-Criado S, Sandberg Y, Langerak AW, González M, Balanzategui A, Orfao A. Expanded cells in monoclonal TCR-alphabeta+/CD4+/NKa+/CD8-/+dim T-LGL lymphocytosis recognize hCMV antigens. Blood 2008; 112(12): 4609-16.

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Infinicyt publication Derolf AR, Björklund E, Mazur J, Björkholm M, Porwit A. Expression patterns of CD33 and CD15 predict outcome in patients with acute myeloid leukemia. Leuk Lymphoma 2008; 49(7): 1279-91.

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Assessment of Infinicyt software-related strategies for flow cytometric immunophenotyping

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Infinicyt publication Hunt AM, Shallenberger W, Ten Eyck SP, Craig, FE. Use of internal control T-cell populations in the flow cytometric evaluation for T-cell neoplasms. Cytometry B Clin Cytom 2016; 90(5): 404-14.

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Infinicyt publication Kumar S, Paiva B, Anderson KC, Durie B, Landgren O, Moreau P, Munshi N, Lonial S, Bladé J, Mateos MV, Dimopoulos M, Kastritis E, Boccadoro M, Orlowski R, Goldschmidt H, Spencer A, Hou J, Chng WJ, Usmani SZ, Zamagni E, Shimizu K, Jagannath S, Johnsen HE, Terpos E, Reiman A, Kyle RA, Sonneveld P, Richardson PG, McCarthy P, Ludwig H, Chen W, Cavo M, Harousseau JL, Lentzsch S, Hillengass J, Palumbo A, Orfao A, Rajkumar SV, San Miguel J, Avet-Loiseau H. International Myeloma Working Group consensus criteria for response and minimal residual disease assessment in multiple myeloma. Lancet Oncol 2016; 17(8): e328-46.

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Infinicyt publication Pedreira CE, Costa ES, Lecrevisse Q, van Dongen JJM, Orfao A; EuroFlow Consortium. Overview of clinical flow cytometry data analysis: recent advances and future challenges. Trends Biotechnol 2013; 31(7): 415-25.

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Infinicyt publication Paiva B, Vídriales MB, Rosiñol L, Martínez-López J, Mateos MV, Ocio EM, Montalbán MÁ, Cordón L, Gutiérrez NC, Corchete L, Oriol A, Terol MJ, Echeveste MA, De Paz R, De Arriba F, Palomera L, de la Rubia J, Díaz-Mediavilla J, Granell M, Gorosquieta A, Alegre A, Orfao A, Lahuerta JJ, Bladé J, San Miguel JF; Grupo Español de MM/Programa para el Estudio de la Terapéutica en Hemopatías Malignas Cooperative Study Group. A multiparameter flow cytometry immunophenotypic algorithm for the identification of newly diagnosed symptomatic myeloma with an MGUS-like signature and long-term disease control. Leukemia 2013; 27(10): 2056-61.

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Infinicyt publication da Costa ES, Peres RT, Almeida J, Lécrevisse Q, Arroyo ME, Teodósio C, Pedreira CE, van Dongen JJM, Orfao A; EuroFlow Consortium. Harmonization of light scatter and fluorescence flow cytometry profiles obtained after staining peripheral blood leucocytes for cell surface-only versus intracellular antigens with the Fix & Perm™ reagent. Cytometry B Clin Cytom 2010; 78(1): 11-20.

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Infinicyt publication Costa ES, Pedreira CE, Barrena S, Lecrevisse Q, Flores J, Quijano S, Almeida J, del Carmen García-Macias M, Bottcher S, van Dongen JJM, Orfao; EuroFlow Consortium. Automated pattern-guided principal component analysis vs expert-based immunophenotypic classification of B-cell chronic lymphoproliferative disorders: a step forward in the standardization of clinical immunophenotyping. Leukemia 2010; 24(11): 1927-33.

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Infinicyt publication Pedreira CE, Costa ES, Arroyo ME, Almeida J Orfao A. A multidimensional classification approach for the automated analysis of flow cytometry data. IEEE Trans Biomed Eng 2008; 55(3): 1155-62.

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Infinicyt publication Pedreira CE, Costa ES, Barrena S, Lecrevisse Q, Almeida J, van Dongen JJM, Orfao A; EuroFlow Consortium. Generation of flow cytometry data files with a potentially infinite number of dimensions. Cytometry A 2008; 73(9): 834-46.

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Infinicyt publication Pedreira CE, Costa ES, Almeida J, Fernandez C, Quijano S, Flores J, Barrena S, Lecrevisse Q, van Dongen JJM, Orfao A; EuroFlow Consortium. A probabilistic approach for the evaluation of minimal residual disease by multiparameter flow cytometry in leukemic B-cell chronic lymphoproliferative disorders. Cytometry A 2008; 73(12): 1141-50.

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